Mass spectrometry (MS) is a reference analytical method used to identify and quantify molecules by measuring mass-to-charge signals, and here it is described as an established orthogonal technique for comparison with nanopore direct RNA sequencing results. It is broadly applied in proteomics, metabolomics, and biomarker detection, including protein/peptide/metabolite profiling for bladder cancer and metabolite analysis from s’wipe-collected stool samples. In tissue regeneration research, MS enabled high-throughput detection of protein signatures and post-translational modifications during healing, highlighting its role in mapping dynamic biological changes. The literature also emphasizes its use as a comparator for RNA quality assessment in cell and gene therapy workflows, underscoring its value as a benchmark analytical platform. Overall, MS remains a versatile orthogonal technology for multi-omics validation, with applications spanning RNA quality control, cancer diagnostics, tissue repair, and gut metabolomics.
Cancer
- MS was mentioned among protein/peptide/metabolite detection approaches for early bladder cancer diagnostics. (PMID:41892063)
- It supports non-invasive urine-based biomarker workflows by enabling molecular detection across multiple analyte classes. (PMID:41892063)
Proteomics and Tissue Regeneration
- MS was used as a high-throughput proteomic technology to identify protein signatures during tissue healing. (PMID:41848587)
- It also detected post-translational modifications, adding mechanistic detail to regeneration studies. (PMID:41848587)
- These findings support MS as a core platform for profiling dynamic protein changes in healing tissues. (PMID:41848587)
RNA Quality Control and Analytical Benchmarking
- MS served as an established orthogonal reference method for comparing RNA quality measurements against nanopore direct RNA sequencing. (PMID:41787951)
- This comparison was applied in the context of mRNA and sgRNA quality assessment for cell and gene therapy applications. (PMID:41787951)
Metabolomics and Multi-omics
- MS was used to assess metabolites captured by s’wipe, an MS-compatible stool collection method. (PMID:41817174)
- The platform supported high-throughput gut metabolomics and multi-omics workflows. (PMID:41817174)